Journal: Scientific Reports

Article Title: The NOP-1 peptide derived from the central regulator of ethylene signaling EIN2 delays floral senescence in cut flowers

doi: 10.1038/s41598-018-37571-x

Figure Lengend Snippet: Molecular binding studies of carnation ethylene receptor DcETR1. ( A ) Microscale Thermophoresis (MST) binding studies using recombinant carnation ethylene receptor DcETR1 and AtEIN2. Titration of unlabeled AtEIN2 to labeled DcETR1 (•) revealed binding of both proteins at a dissociation constant (K D ) of 135 nM ± 0.35 nM, indicating a tight interaction between DcETR1 and AtEIN2. ( B ) MST binding studies on DcETR1 and NOP-1. Titration of unlabeled NOP-1 to labeled DcETR1 (•) resulted in a K D of 3.49 µM ± 0.55 µM. Asterisks (*) indicate the labeling of DcETR1 with AlexaFluor488-NHS. Negative controls using chemically denatured DcETR1 shows no interaction with the EIN2 protein or the NOP-1 peptide (•).

Article Snippet: According to the published sequence (Carnation DB: Dca62022.1), full length codon optimized cDNA sequence encoding Dianthus caryophyllus ethylene receptor DcETR1 was ordered at GenScript United States.

Techniques: Binding Assay, Microscale Thermophoresis, Recombinant, Titration, Labeling

Journal: Biosafety and Health

Article Title: Molecular phylogenomic analysis reveals a single origin of monkeypox virus transmission in Guangzhou, China in June 2023

doi: 10.1016/j.bsheal.2023.08.002

Figure Lengend Snippet: Phylogenetic analysis of monkeypox (mpox) virus sequences first reported in Guangzhou, China. A) The eight local sequences (red dot) in FASTA format were aligned by the BioEdit software 7.0.9 with the reference sequences of mpox viral clade IIb downloaded from GISAID (black dot) and NCBI (NC_063383.1, green dot, selected as the outgroup). The maximum likelihood (ML) phylogenetic tree of the full-length genome sequences of mpox virus was constructed with the MEGA software 11.0.11 using the General Time Reversible model with 500 bootstrap replicates. The scale bar represents the genetic distance. Bootstrap values >75% are presented at the corresponding nodes of the tree. Abbreviations: FASTA, Fast-All; GISAID, Global Initiative on Sharing Avian Influenza Data; NCBI, National Center For Biotechnology Information. B) The analysis of amino acid mutation of the eight local sequences compared to the reference Japan|EPI ISL 17692269|2023-04-28. In most cases, the mutation sites are mixed. Only the dominant amino acid is shown. “*” represents termination codon. As for this special site, the CAG (Q) codon accounts for 29.26% of all reads, but the TAG (stop) codon accounts for 70.74%. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: We then performed the amplicon-based mpox viral full-length genome sequencing of mpox virus using a newly developed method by MGI Tech Co., Ltd. (MGI), China.

Techniques: Virus, Software, Construct, Mutagenesis